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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
29/06/2021 |
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Data da última atualização: |
01/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AZEVEDO, B. T.; VERCESI FILHO, A. E.; GUTMANIS, G.; VERISSIMO, C. J.; KATIKI, L. M.; OKINO, C. H.; OLIVEIRA, M. C. de S.; GIGLIOTI, R. |
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Afiliação: |
BIANCA TAINA AZEVEDO, IZ; ANIBAL EUGENIO VERCESI FILHO, IZ; GUNTA GUTMANIS, IZ; CECÍLIA JOSE VERISSIMO, IZ; LUCIANA MORITA KATIKI, IZ; CINTIA HIROMI OKINO, CPPSE; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; RODRIGO GIGLIOTI, IZ. |
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Título: |
New sensitive methods for fraud detection in buffalo dairy products. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
International Dairy Journal, v.117, 105013, jun. 2021. |
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Páginas: |
9 p. |
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DOI: |
https://doi.org/10.1016/j.idairyj.2021.105013 |
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Idioma: |
Inglês |
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Conteúdo: |
The rising consumption of buffalo milk derivatives in recent years has highlighted the search for buffaloes in Brazilian livestock farming. These products have greater added value compared with common dairy milk products. Consequently, addition of variable amounts of cows' milk during manufacture of buffalo dairy products may occur, which constitutes fraud by product adulteration. Thus, the present study developed and standardised a DNA extraction protocol for application on different dairy derivatives and two methods based on real-time PCR for fraud identification: high-resolution melting (HRM) and rhAmp® SNP system. The extraction method allowed to extraction of DNA from eleven different dairy products. The sensitivity of the rhAmp method (1%) was five times higher than HRM assay (5%) and may be considered a better choice for identification of product adulteration when high sensitivity levels are required. |
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Palavras-Chave: |
DNA extraction; High resolution melting; Real time PCR; RhAmpR SNP system. |
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Thesaurus Nal: |
Buffalo milk. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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001 2132629
005 2021-07-01
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.idairyj.2021.105013$2DOI
100 1 $aAZEVEDO, B. T.
245 $aNew sensitive methods for fraud detection in buffalo dairy products.$h[electronic resource]
260 $c2021
300 $a9 p.
520 $aThe rising consumption of buffalo milk derivatives in recent years has highlighted the search for buffaloes in Brazilian livestock farming. These products have greater added value compared with common dairy milk products. Consequently, addition of variable amounts of cows' milk during manufacture of buffalo dairy products may occur, which constitutes fraud by product adulteration. Thus, the present study developed and standardised a DNA extraction protocol for application on different dairy derivatives and two methods based on real-time PCR for fraud identification: high-resolution melting (HRM) and rhAmp® SNP system. The extraction method allowed to extraction of DNA from eleven different dairy products. The sensitivity of the rhAmp method (1%) was five times higher than HRM assay (5%) and may be considered a better choice for identification of product adulteration when high sensitivity levels are required.
650 $aBuffalo milk
653 $aDNA extraction
653 $aHigh resolution melting
653 $aReal time PCR
653 $aRhAmpR SNP system
700 1 $aVERCESI FILHO, A. E.
700 1 $aGUTMANIS, G.
700 1 $aVERISSIMO, C. J.
700 1 $aKATIKI, L. M.
700 1 $aOKINO, C. H.
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aGIGLIOTI, R.
773 $tInternational Dairy Journal$gv.117, 105013, jun. 2021.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 5 | |
| 2. |  | GIGLIOTI, R.; OKINO, C. H.; AZEVEDO, B. T.; GUTMANIS, G.; KATIKI, L. M.; OLIVEIRA, M. C. de S.; VERCESI FILHO, A. E. Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples. Food Chemistry: Molecular Sciences, v.3, 2021, 100055. 6 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 3. | | AZEVEDO, B. T.; VERCESI FILHO, A. E.; GUTMANIS, G.; VERISSIMO, C. J.; KATIKI, L. M.; OKINO, C. H.; OLIVEIRA, M. C. de S.; GIGLIOTI, R. New sensitive methods for fraud detection in buffalo dairy products. International Dairy Journal, v.117, 105013, jun. 2021. 9 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|  |
| 4. | | GIGLIOTI, R.; OKINO, C. H.; AZEVEDO, B. T.; WEDY, B. C. R.; GUTMANIS, G.; VERÍSSIMO, C. J.; KATIKI, L. M.; VERCESI FILHO, A. E.; OLIVEIRA, H. N. DE; OLIVEIRA, M. C. de S. Semi-quantitative evaluation of Babesia bovis and B. bigemina infection levels estimated by HRM analysis. Ticks and Tick-borne Diseases, v.12, n.5, 101753, sep. 2021. 6 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 5. | | GIGLIOTI, R.; OLIVEIRA, H. N. de; GUTMANIS, G.; LUCIANI, G. F.; AZEVEDO, B. T.; FIORIN, C. F. de C.; ANDRADE, M. F. de; SILVA, M. A. F.; VERCESI FILHO, A. E.; KATIKI, L. M.; OKINO, C. H.; OLIVEIRA, M. C. de S.; VERÍSSIMO, C. J. Correlations and repeatability between Babesia spp. infection levels using two dairy cattle breeding systems. Experimental and Applied Acarology, v. 81, p. 599-607, jul. 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 5 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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